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purification of plant viruses

Purification of Plant Viruses Nature

Purification of Plant Viruses D. MACCLEMENT Nature 133,760 ( 1934) Cite this article 406 Accesses 6 Citations Metrics Abstract THE following is a

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Purification of Plant Viruses Springer Nature Experiments

Purified virus is required for the production of antibodies, physical, biochemical and molecular characterization of virus isolates. Purification of virus involves several steps such as propagation of the virus in the host, extraction of sap, clarification, concentration and further purification.

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The Purification of Plant Viruses ScienceDirect

procedures 1 in the purification plant of viruses in order to obtain preparations of purified virus, these steps should be followed: (1) the most suitable plants should be used and should be grown, inoculated, and harvested under optimum conditions; (2) the most suitable * after the manuscript for this ohapter waa submitted a paper has appeared

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Purification of Plant Viruses ScienceGate

Read Purification of Plant Viruses. Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to

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Purification of plant viruses and virus coat proteins by high

High performance liquid chromatography (HPLC) gel filtration has been successfully applied in the purification of elongated and isometric plant viruses. Two different approaches have been tested. In one approach, semi-purified virus particles were dissociated with lithium chloride and the released coat proteins purified by HPLC gel filtration.

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[PDF] Purification of Plant Viruses Semantic Scholar

Purification of Plant Viruses. D. Macclement. Published 1 May 1934. Biology. Nature. THE following is a practical method of preparing a purified suspension of any of the ‘x’ group of plant viruses, from the extracted juice of the diseased plant. View

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Isolation and Purification of Plant Viruses

Isolation and Purification of Plant Viruses Kenneth M. Smith C.B.E., D.Sc., Ph.D., F.R.S. Chapter 76 Accesses Abstract When investigating a possibly undescribed sap-transmissible virus, it is desirable to have some information on certain

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Purification of plant viruses by zone electrophoresis

vieology 23, 495-502 (1964) purification of plant viruses by zone electrophoresis m. h. v. van regenmortel department of microbiology, university of stellenbosch, south africa accepted april 16, 196^ the purification of plant viruses by a final step of zone electrophorcsis is advo- cated as one of the most versatile and efficient methods of

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Purification of plant viruses and virus coat proteins by high

1990-6-1  HPLC of lithium chloride-treated virus Virus purified by the basic procedures described above was diluted to 5-20 mg/ml with distilled water, mixed with an equal volume of 4 M lithium chloride and kept at -20 for 48-72 h. This procedure has previously been found to precipitate virus RNA, releasing soluble coat protein (Moghal and Francki, 1976).

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The Purification of Plant Viruses ScienceDirect

This chapter presents an outline on the purification of plant viruses and discusses the methods of purification. Appropriate steps toward purification are described in detail both for those viruses that have yet to be purified and for those that have already been obtained as relatively monodisperse suspensions but for which there is interest in securing the highest degree of

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Purification of Plant Viruses ScienceGate

Read Purification of Plant Viruses. Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to

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[PDF] Purification of Plant Viruses Semantic Scholar

Purification of Plant Viruses. D. Macclement. Published 1 May 1934. Biology. Nature. THE following is a practical method of preparing a purified suspension of any of the ‘x’ group of plant viruses, from the extracted juice of the diseased plant. View

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Purification of Plant Viruses Request PDF

2020-3-6  In book: Characterization of Plant Viruses (pp.133-168) Authors:

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The Purification of Plant Viruses ScienceDirect

This chapter presents an outline on the purification of plant viruses and discusses the methods of purification. Appropriate steps toward purification are described in detail both for those viruses that have yet to be purified and for those that have already been obtained as relatively monodisperse suspensions but for which there is interest in securing the highest degree of

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Purification of plant viruses by zone electrophoresis

The purification of plant viruses by a final step of zone electrophoresis is advocated as one of the most versatile and efficient methods of obtaining

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Purification of plant viruses and virus coat proteins by high

1990-6-1  High performance liquid chromatography (HPLC) gel filtration has been successfully applied in the purification of elongated and isometric plant viruses. Two different approaches have been tested. In one approach, semi-purified virus particles were dissociated with lithium chloride and the released coat proteins purified by HPLC gel filtration.

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Purification of filamentous plant viruses by thin layer

The construction of a modified thin layer ultracentrifuge rotor is described. This rotor was used in the purification of five filamentous plant viruses, viz. TMV, SCMV, PVX, SGV and YMC. The purification and concentration of these viruses in their monomeric forms is hazardous when conventional "tube

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Isolation and Purification SpringerLink

The isolation and purification of plant viruses are necessary for the initial identification and characterization of viruses, for the production of antisera, and for a multitude of other studies. The terms “isolation” and “purification” often are used interchangeably by virologists to describe separation of virus particles in a pure

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Purification of Viruses SpringerLink

Abstract. Each virus poses an individual purification problem that is related to the properties of the virus, the nature of the host, and the culture conditions. Consequently, it is not possible to outline a purification procedure that will work with equal effectiveness for all viruses. Nevertheless, it is possible to describe a few methods and

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The Purification of Plant Viruses ScienceDirect

This chapter presents an outline on the purification of plant viruses and discusses the methods of purification. Appropriate steps toward purification are described in detail both for those viruses that have yet to be purified and for those that have already been obtained as relatively monodisperse suspensions but for which there is interest in securing the highest degree of

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Purification of Plant Viruses ScienceGate

Read Purification of Plant Viruses. Schumacher and Halbsguth first demonstrated ectodesmata as pores or channels in the epidermal cell walls in haustoria of Cuscuta odorata L. by light microscopy in tissues fixed in a sublimate fixative (30% ethyl alcohol, 30 ml:glacial acetic acid, 10 ml: 65% nitric acid, 1 ml: 40% formaldehyde, 5 ml: oxalic acid, 2 g: mecuric chloride to

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[PDF] Purification of Plant Viruses Semantic Scholar

Purification of Plant Viruses. D. Macclement. Published 1 May 1934. Biology. Nature. THE following is a practical method of preparing a purified suspension of any of the ‘x’ group of plant viruses, from the extracted juice of the diseased plant. View

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Purification of Plant Viruses Request PDF

2020-3-6  In book: Characterization of Plant Viruses (pp.133-168) Authors:

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The Purification of Plant Viruses ScienceDirect

This chapter presents an outline on the purification of plant viruses and discusses the methods of purification. Appropriate steps toward purification are described in detail both for those viruses that have yet to be purified and for those that have already been obtained as relatively monodisperse suspensions but for which there is interest in securing the highest degree of

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The Purification of Plant Viruses Semantic Scholar

This chapter presents an outline on the purification of plant viruses and discusses the methods of purification and there are indications that nucleic acid obtained from plant viruses is infective by itself. Publisher Summary This chapter presents an outline on the purification of plant viruses and discusses the methods of purification. Appropriate steps toward purification are described in

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Isolation and Purification of Plant Viruses

Isolation and Purification of Plant Viruses. Authors; Authors and affiliations; Kenneth M. Smith; Chapter. 253 Downloads; Abstract. When investigating a possibly undescribed sap-transmissible virus, it is desirable to have some

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Purification of filamentous plant viruses by thin layer

The construction of a modified thin layer ultracentrifuge rotor is described. This rotor was used in the purification of five filamentous plant viruses, viz. TMV, SCMV, PVX, SGV and YMC. The purification and concentration of these viruses in their monomeric forms is hazardous when conventional "tube

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Purification of Plant Viruses NASA/ADS

Purification of Plant Viruses Macclement, D. Abstract. THE following is a practical method of preparing a purified suspension of any of the `x' group of plant viruses, from the extracted juice of the diseased plant. Publication: Nature. Pub Date: May 1934 DOI: 10.1038/133760b0 Bibcode: 1934Natur.133Q.760M

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Purification of viruses by electrophoresis in sucrose

Abstract. Zone electrophoresis (ZE) in concentration gradients of sucrose proved to be a powerful technique in the purification of viruses although it is seldomly used as a single step for the separation of the infective agent from extraneous matter but usually as a final procedure. No difficulties were experienced in the purification of plant

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